Coding

Part:BBa_K1458005:Design

Designed by: Shani Gal-Oz   Group: iGEM14_BGU_Israel   (2014-10-15)


Human DsbA-L (GSTK1) gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 508
    Illegal SapI site found at 27


Design Notes

The DsbA-L sequence had iGEM Restriction sites that had to be change according to iGEM protocol. In position 131 G was changed to A to avoid Pst1 Site. In position 446 A was changed to T to avoid Pst1 Site. In position 467 A was changed to T to avoid Pst1 Site. In position 545 C was changed to G to avoid Pst1 Site. All changes in nucleotides was done according to an amino acid chart to maintain the same amino acids at the protein sequence Level.



Source

Human GSTK1 on NCBI : http://www.ncbi.nlm.nih.gov/gene/373156

References